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61.
Clive A. Henrick Barbara A. Garcia Gerardus B. Staal David C. Cerf Richard J. Anderson Karen Gill Henry R. Chinn Jeffrey N. Labovitz Michael M. Leippe Sam L. Woo Robert L. Carney Douglas C. Gordon Gustave K. Kohn 《Pest management science》1980,11(2):224-241
A new series of substituted 2-anilino-3-methylbutyrates has been prepared; bioassay data for these compounds on Heliothis virescens, Musca domestica, Aphis fabae and Tetranychus urticae are presented and discussed. Some unexpected relationships were observed between the nature of the substituents and the biological activity. Increases in foliar stability were noted with certain substitution patterns. Both α-cyano-3-phenoxybenzyl 3-methyl-2-(α, α, α,2-tetrafluoro-p-toluidino)butyrate and the corresponding 2-(2-chloro-α, α, α-trifluoro-p-toluidino)-3-methylbutyrate showed good stability in air and light, and exhibited biological activities of a similar nature and potency to those of previously known synthetic pyrethroids. Esters of the (R)-2- anilino-3-methylbutyric acids are far more active than those prepared from the (S)-enantiomers. The (R)-configuration at C-2 in these acids is sterically equivalent to the active absolute configuration at the chiral carbon α to the carboxylate group in both the permethrin and the fenvalerate types of pyrethroids. A new class of insecticidal 2-(isoindolin-2-yl)alkanoates is also reported. In this series the most biologically active analogue was α-cyano-3-phenoxybenzyl 3-methyl-2-(4,5,6,7-tetrafluoroisoindolin-2-yl)butyrate. These esters were considerably less stable than the anilino analogues on exposure to air and light. 相似文献
62.
M T Fox D Gerrelli S R Pitt D E Jacobs M Gill A D Simmonds 《Research in veterinary science》1989,47(3):299-304
Metabolic effects of a trickle challenge with the equivalent of 10,000 infective Ostertagia ostertagi larvae per day were investigated in 12 calves allocated to infected, pair-fed control or ad libitum-fed control groups. Changes in hormone levels reflecting abomasal, pituitary and pancreatic function were monitored using radioimmunoassay techniques previously validated for use in cattle. A range of metabolic profile parameters and blood metabolites was also measured. Feed intake of the infected calves began to decline as blood gastrin and pepsinogen levels reached a peak. The depression in appetite recorded in this group was responsible for significant increases in plasma urea and non-esterified fatty acid levels and associated with an increase in growth hormone/insulin ratio. No significant difference in glucagon levels was recorded between groups. A decline in blood albumin values was also shown in the infected group and associated with a drop in nitrogen digestibility. A significant depression in circulating calcium levels was related to either the hypoalbuminaemia or impaired mineral absorption in the intestine. A decrease in plasma cholesterol values in the infected group was associated with changes in digestive function. 相似文献
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A mathematical model of lactating sow metabolism was evaluated using three types of tests. First, 16 experimental treatments from four experiments reported in the literature were simulated with the model, and the simulated values for change in BW and protein and fat content were compared to reported or calculated values. Second, the model's response to level of feed intake, level of milk production, BW and composition at farrowing, and dietary lysine concentration was compared to expected responses. Third, the model's sensitivity to changes in several of its kinetic parameters was measured. There was good agreement between simulated and measured values for BW and fat loss and reasonable agreement for body protein loss. All responses to changes in external conditions were in expected directions and biologically reasonable. The model seemed rather robust with respect to changes in the kinetic parameters considered, although important changes in simulated values were found in some cases. Overall, the model seems sound. It can be useful in evaluation of feeding programs and in understanding biological relationships. 相似文献
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Callus tissue cultures have been established from the excised segments of the inflorescence, flower stalks, denuded flower, bract, perianth and leaf segments of 2 cultivars of Gladiolus grandiflorus. Of all the explants and the media tested, the best callus was obtained from the segments of the flower stalks, cultured on a basal medium supplemented with naphthalene acetic acid and kinetin. The callus mostly underwent rhizogenesis, and occasionally differentiated shoots. Complete plants were regenerated from the in vitro cultured cormels, cormel tips and the axillary buds, and 6 plants were formed from the segments of 1 cormel, whereas in nature only 1 plant is obtained per cormel. Cultured young anthers callused and developed leaf-like petaloid structures, and occasionally showed multicellular pollen. 相似文献
67.
Groups of cattle were inoculated subcutaneously with (i) a recombinant DNA-derived Babesia bovis protein (KaBbl-GZ) fused to beta-galactosidase and combined with adjuvants, or (ii) native beta-galactosidase (GZ) plus adjuvant, or (iii) adjuvant only or (iv) a live, attenuated B bovis vaccine. KaBbl-GZ was produced in the lambda gt11-amp3 system as a 5-10 kD babesial polypeptide linked to GZ. KaBbl has previously been shown to be an immunodominant antigen of B bovis, localised at the apex of the parasite, and present in a range of B bovis strains. High levels of GZ antibodies were observed in KaBbl-GZ and GZ inoculated cattle, but specific KaBbl antibodies could not be detected by ELISA. Five months after primary inoculation, all cattle were blood challenged with a virulent heterologous B bovis strain. Despite four inoculations with KaBbl-GZ, significant protection against the challenge was not observed. 相似文献
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Strip loins from two experiments were used to evaluate effects of feeding dry (DDG) or wet (WDG) distiller's grains on beef color, tenderness, and sensory traits of Holstein steers. In Exp. 1, conducted at the University of Illinois at Champaign-Urbana, dietary treatments consisted of a control whole corn-corn silage diet with soybean meal (SBM) or diets formulated with 12.5% DDG plus urea, 25% DDG, 25% WDG, 50% DDG, or 50% WDG (DM basis). In Exp. 2, conducted at Iowa State University, dietary treatments consisted of cracked corn-corn silage-hay diets with either SBM or urea (Urea) as the control diets, or diets formulated with 10, 20, or 40% DDG or WDG (DM basis). Within each study, strip loins from each of four steers (representing 45.7 and 66.6% of steers in Exp. 1 and 2, respectively) in four replicate pens per treatment were aged for 13 d at 4 degrees C for subsequent color, tenderness, and palatability evaluation. Color of steaks was measured objectively using a HunterLab Miniscan XE spectrophotometer and was subjectively evaluated by a trained panel. Tenderness was measured using the Warner-Bratzler shear force (WBSF) instrument on steaks cooked to 70 degrees C. For sensory evaluation, 95 consumers were recruited to evaluate tenderness, juiciness, and flavor of cooked steaks. In Exp. 1, steaks from steers fed 25% WDG had higher (P < 0.05) a* values after 138 h of simulated retail display than all other treatments, except for those from steers fed 12.5% DDG. In Exp. 2, a greater (P < 0.05) percentage of steaks from steers fed 40% DDG or 40% WDG were considered moderately undesirable during retail display (steaks that received a consumer acceptability score of 3 or less). There were no (P = 0.20 in Exp. 1, and P = 0.33 in Exp. 2) differences among treatments in Exp. 1 and Exp. 2 for WBSF (1.47 +/- 0.66 kg and 1.58 +/- 0.72 kg, respectively) or taste panel tenderness (5.7 +/- 0.30 and 6.2 +/- 0.22, respectively), beef flavor (6.0 +/- 0.23 and 6.2 +/- 0.22, respectively), and juiciness (5.6 +/- 0.31 and 5.8 +/- 0.23). Feeding distiller's grains at up to 50% of the dietary DM did not affect tenderness or sensory traits, and seems to be a viable feed alternative without negatively impacting sensory attributes. 相似文献